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Molecular Parasitology and
Protein Engineering

 

 





1.  Sourav Roy, Lakshmeesha K. Nagappa, Vasudeva S. P. and Hemalatha Balaram,  Kinetic mechanism of Plasmodium falciparum hypoxanthine-guanine-xanthine phosphoribosyltransferase, Molecular and Biochemical Parasitology,  204, 111 - 120 (2015).

2.  Sourav Roy, Tarak Karmakar, Vasudeva S. P., Lakshmeesha K. Nagappa, Sundaram Balasubramanian and Hemalatha Balaram,  Slow ligand-induced conformational switch increases the catalytic rate in Plasmodium falciparum hypoxanthine guanine xanthine phosphoribosyltransferase, Molecular Biosystems,  11, 1410 - 1424 (2015).

3.  Bharath Srinivasan, Lakshmeesha Kempaiah Nagappa, Arpit Shukla and Hemalatha Balaram,  Prediction of substrate specificity and preliminary kinetic characterization of the hypothetical protein PVX_123945 fromPlasmodium vivax, Experimental Parasitology,  151, 56 - 63 (2015).

4.  Lionel Ballut, Sebastien Violot, Santosh Shivakumaraswamy, Lakshmi Prasoona Thota, Manu Sathya, Jyothirmai Kunala, Bauke W. Dijkstra, Raphael Terreux, Richard Haser, Hemalatha Balaram and Nushin Aghajari,  Active site coupling in Plasmodium falciparum GMP synthetase is triggered by domain rotation, Nature Communications,  6, (2015).

5.  Bharath Srinivasan, Farhad Forouhar, Arpit Shukla, Sonia Kulkarni, Chethana Sampangi and Hemalatha Balaram,  Allosteric regulation and substrate activation in cytosolic nucleotidase-II from Legionella pneumophila, FEBS J,  (2014).

6.  Rustam Ali, Sanjeev Kumar, Hemalatha Balaram and Siddhartha Sarma,  Solution Nuclear Magnetic Resonance Structure of the GATase Subunit and Structural Basis of the Interaction between GATase and ATPPase Subunits in a two-subunit-type GMPS from Methanocaldococcus jannaschii, Biochemistry,  52, 4308 - 4323 (2013).

7.  Bhat, J. Y., Venkatachala, R. and Balaram, H.,  Substrate-induced conformational changes in Plasmodium falciparum guanosine monophosphate synthetase, FEBS J,  278, 3756 - 3768 (2011).

8.  Bhat, J. Y., Venkatachala, R., Singh, K., Gupta, K., Sarma, S. P. and Balaram, H.,  Ammonia Channeling in Plasmodium falciparum GMP Synthetase: Investigation by NMR Spectroscopy and Biochemical Assays, Biochemistry,  50, 3346 - 3356 (2011).

9.  Vinay Bulusu, Vijay Jayaraman and Hemalatha Balaram,  Metabolic Fate of Fumarate, a Side Product of the Purine Salvage Pathway in the Intraerythrocytic Stages of Plasmodium falciparum, J Biol Chem,  286, 9236 - 9245 (2011).

10.  Chakrabarty SP, Ramapanicker R, Mishra R, Chandrasekaran S and Balaram H Development and characterization of lysine based tripeptide analogues as inhibitors of Sir2 activity., Bioorg Med Chem,  17, 8060 - 8072 (2009).

11.  Bulusu V, Srinivasan B, Bopanna M P and Balaram H Elucidation of the substrate specificity, kinetic and catalytic mechanism of adenylosuccinate lyase from Plasmodium falciparum, Biochim Biophys Acta.,  1794, 642 - 654 (2009). , Read abstract.

12.  Mehrotra S and Balaram H Methanocaldococcus jannaschii adenylosuccinate synthetase: Studies on temperature dependence of catalytic activity and structural stability, Biochim Biophys Acta.,  1784, 2019 - 2028 (2008). , Read abstract.

13.  Datta KKR, Srinivasan B, Balaram H and Eswaramoorthy M,  Synthesis of agarose-metal/semiconductor nanoparticles having superior bacteriocidal activity and their simple conversion to metal-carbon composites, journal of chemical sciences,  120, 579 - 586 (2008). pdf , Read abstract.

14.  Gayathri P, Sujay Subbyya IN, Ashok CS, Selvi TS, Balaram H and Murthy MRN,  Crystal structure of a chimera of human and Plasmodium falciparum hypoxanthine guanine phosphoribosyltransferases provides insights into oligomerization, Proteins,  73, 1010 - 1020 (2008). , Read abstract.

15.  Chakrabarty SP, Saikumari YK, Bopanna MP and Balaram H  Biochemical characterization of Plasmodium falciparum Sir2, a NAD+-dependent deacetylase, Molecular and Biochemical Parasitology,  158, 139 - 151 (2008). , Read abstract.

16.  Bhat JY, Shastri BG and Balaram H Kinetic and biochemical characterization of Plasmodium falciparum GMP synthetase., Biochemical journal,  409, 263 - 273 (2008). , Read abstract.

17.  Gayathri P, Balaram H and Murthy MRN,  Structural biology of plasmodial proteins, current opinion in structural biology,  17, 744 - 754 (2007). , Read abstract.

18.  Mehrotra S and Balaram H  Kinetic characterization of adenylosuccinate synthetase from the thermophilic archaea Methanocaldococcus jannaschii, Biochemistry,  46, 12821 - 12832 (2007). , Read abstract.

19.  Srinivasan B and Balaram H ISN1 nucleotidases and HAD superfamily protein fold: in silico sequence and structure analysis, In silico Biology,  7, 0019 (2007). , Read abstract.

20.  Sabareesh V, Ranganayaki RS, Raghothama S, Bopanna MP, Balaram H, Srinivasan MC and Balaram P,  Identification and characterization of a library of microheterogeneous cyclohexadepsipeptides from the fungus Isaria, journal of natural products,  70, 715 - 729 (2007). , Read abstract.

21.  R. Jayalakshmi and H. Balaram,  Nucleotide metabolism in Plasmodium falciparum: recent developments, Medicinal Chemistry Reviews,  1, 465 - 473 (2004).

22.  R. Jayalakshmi, Sonali Mehrotra, Ranjith P. Anand and H. Balaram,  Unique kinetic mechanism of Plasmodium falciparum adenylosuccinate synthetase, Molecular and Biochemical Parasitology,  138, 1 - 8 (2004).

23.  R. Jayalakshmi, K. Sumathy, R. P. Anand and H. Balaram,  A non-active site mutation in human hypoxanthine guanine phosphoribosyltransferase expands substrate specificity, Archives of Biochemistry and Biophysics,  427, 116 - 122 (2004).

24.  K. Eaazhisai, R. Jayalakshmi, P. Gayathri, R. P. Anand, K. Sumathy, H. Balaram and M. R. N. Murthy,  Crystal structure of fully-ligated adenylosuccinate synthetase from Plasmodium falciparum, Journal of Molecular Biology,  335, 1251 - 1264 (2004).

25.  S. Parthasarathy, K. Eaazhisai, H. Balaram, P. Balaram and M. R. N. Murthy,  Structure of Plasmodium falciparum TIM-2-phosphoglycerate complex at 1.1A resolution, Journal of Biological Chemistry,  278, 52461 - 52470 (2003).

26.  H. N. Gopi, G. Ravindra, P. P. Pal, P. Pattanaik, H. Balaram and P. Balaram,  Proteolytic stability of beta-peptide bonds probed using quenched fluorescent substrates incorporating a hemoglobin cleavage site, FEBS Letters,  535, 175 - 178 (2003).

27.  P. Pattanaik, G. Ravindra, C. Sengupta, K. Maithal, P. Balaram and H. Balaram,  Unusual fluorescence of W168 in Plasmodium falciparum triosephosphate isomerase, probed by single-tryptophan mutants, European Journal of Biochemistry,  270, 745 - 756 (2003).

28.  P. Pattanaik, B. Jain, G. Ravindra, H. N. Gopi, P. P. Pal, H. Balaram and P. Balaram,  Stage specific profiling of Plasmodium falciparum proteases using an internally quenched multispecificity protease substrate, Biochemical and Biophysics Research Communications,  309, 974 - 979 (2003).

29.  Kapil Maithal, G. Ravindra, G. Nagaraj, S. Kumar Singh, H. Balaram and P. Balaram,  Subunit interface mutation disrupting an aromatic cluster in Plasmodium falciparum triosephosphate isomerase: effect on dimer stability, Protein Engineering,  15, 575 - 584 (2002).

30.  I. N. Sujay Subbayya and H. Balaram,  A Point mutation at the subunit interface of hypoxanthine-guanine-xanthine phosphoribosyltransferase impairs activity: Role of oligomerization in catalysis, FEBS Letters,  521, 72 - 76 (2002).

31.  P. Pattanaik, R. Jayalakshmi and H. Balaram,  Perspectives in drug design against malaria, Current Topics in Medicinal Chemistry,  2, 483 - 505 (2002).

32.  R. Jayalakshmi, K. Sumathy and H. Balaram,  Purification and characterization of recombinant Plasmodium falciparum adenylosuccinate synthetase expressed in Escherichia coli, Protein Expression and Purification,  25, 65 - 72 (2002).

33.  S. K. Singh, K. Maithal, H. Balaram and P. Balaram,  Synthetic peptides as inactivators of multimeric enzymes: inhibition of Plasmodium falciparum triosephosphate isomerase by interface peptides, FEBS Letters,  501, 19 - 23 (2001).

34.  G. Nagaraj, M.V.Uma, M. S. Shivayogi and H. Balaram,  Antimalarial activities of peptide antibiotics isolated from fungi, Antimicrobial Agents and Chemotherapy,  45, 145 - 149 (2001).

35.  K. Shivashankar, I. N. S. Subbayya and H. Balaram,  Development of a bacterial screen for novel hypoxanthine-guanine phosphoribosyltransferase substrates, Journal of Molecular Microbiology and Biotechnology,  3, 557 - 662 (2001).

36.  K. Sumathy, R. Jayalakshmi, M. S. Shivayogi and H. Balaram,  Cloning and characterization of the Plasmodium falciparum adenylosuccinate synthetase gene, Current Science,  78, 101 - 105 (2000).

37.  I. N. S. Subbayya, S. Sukumaran, K. Shivashankar and H. Balaram,  Unusual substrate specificity of a chimeric hypoxanthine-guanine phosphoribosyltransferase containing segments from the Plasmodium falciparum and human enzymes, Biochemical and Biophysical Research Communications,  272, 596 - 602 (2000).

38.  I. N. S. Subbayya and H. Balaram,  Evidence of multiple active states of Plasmodium falciparum Hypoxanthine-guanine-xanthine phosphoribosyltransferase, Biochemical and Biophysical Research Communications,  279, 433 - 437 (2000).

39.  P. Gopal, S. S. Ray, R. Ghokale, H. Balaram, M. R. N. Murthy and P. Balaram,  Cavity creating mutation at the dimer interface of Plasmodium falciparum triosephosphate isomerase: restoration of stability by disulfide cross-linking of subunits, Biochemistry,  38, 478 - 486 (1999).

40.  R. S. Ghokale, S. S. Ray, H. Balaram and P. Balaram,  Unfolding of Plasmodium falciparum triosephosphate isomerase in urea and guanidinium chloride. Evidence for a novel disulfide exchange reaction in a covalently cross-linked mutant, Biochemistry,  38, 423 - 431 (1999).

41.  H. Balaram and I. N. S. Subbayya,  Tinkering with enzymes, Journal of Indian Institute of Science,  79, 49 - 60 (1999).

42.  S. S. Ray, H. Balaram and P. Balaram,  Unusual stability of a multiply nicked form of Plasmodium falciparum triosephosphate isomerase, Chemistry and Biology,  6, 625 - 637 (1999).

43.  I. N. S. Subbayya, S. S. Ray, P. Balaram and H. Balaram,  Metabolic enzymes as potential drug targets in Plasmodium falciparum, Indian Journal of Medical Research,  106, 79 - 94 (1997).

44.  S. S. Velanker, S. S. Ray, R. Ghokale, S. Suma, H. Balaram, P. Balaram and M. R. N. Murthy,  Triosephosphate isomerase from Plasmodium falciparum: the crystal structure provides insights into antimalarial drug design, Structure,  5, 751 - 761 (1997).

45.  J. Ranie, V. P. Kumar and H. Balaram,  Cloning of the triosephosphate isomerase gene of Plasmodium falciparum and expression in Escherichia coli, Molecular and Biochemical Parasitology,  61, 159 - 169 (1993).