Intact in situ Preparation of Drosophila melanogaster Lymph Gland for Comprehensive Analysis of Larval Hematopoiesis - Bio-protocol, 2021
Intact in situ Preparation of Drosophila melanogaster Lymph Gland for Comprehensive Analysis of Larval Hematopoiesis - Bio-protocol, 2021
In this study, we provide a detailed protocol for larval fillet preparation to access and analyze the anterior and posterior lymph gland lobes along with the dorsal vessel and pericardial cells. We demonstrate that tissue architecture and integrity is maintained and provide methods of quantitative analysis. This protocol can be used to quickly and effectively isolate complete LGs from first instar larval to pupal stages and can be implemented with ease. We have used this protocol for a comprehensive analysis of Drosophila hematopoiesis.
