Allostery and ammonia tunnelling in GMP synthetase, a glutamine amidotransferase.
The final step in GMP synthesis from IMP is catalyzed by guanosine monophosphate synthetase (GMPS). The hallmark of this enzyme function involves domain cross-talk, allostery and ammonia tunnelling. These have addressed using a range of techniques including enzyme kinetics, NMR spectroscopy, X-ray crystallography, H/D exchange, etc.
a.H/DX mapped onto the modelled structure of PfGMPS
b.Comparison of one-dimensional 15N-edited proton NMR spectra of glutamine and PfGMP synthetase- catalyzed reaction
1. Ballut L, Violot S, Shivakumaraswamy S, Thota L P, Sathya M, Kunala J, Dijkstra B W, Terreux R, Haser R, Balaram H and Aghajari N.
