Molecular enzymology and protein structure function analysis.
Molecular enzymology and protein structure function analysis.
Enzymes under study have been human and P. falciparum HGPRT P. falciparum and Methanocaldococcus jannaschii adenylosuccinate synthetase, Legionella pneumophila cN-II, M. jannaschii GMPS, triosephosphate isomerase from different organisms using various tools of biochemistry, structural biology, Molecular Dynamics simulation, mass spectrometry, UV Resonance Raman spectroscopy.
N109 in M. jannaschii GATase is modified to succinimide
a.General mechanism for succinimide (Su) formation and hydrolysis
b.LC-ESI-MS of WT MjGATase (Mcalc 21,020 Da). Inset: deconvoluted spectrum. 21,003 Da corresponds to WTSu